Volume: 1 Issue: 1
Year: 2024, Page: 16-22, Doi: https://doi.org/10.71325/ajjms.v1i1.9
Received: Sept. 6, 2024 Accepted: Dec. 5, 2024 Published: Dec. 30, 2024
Introduction and Aim: Microorganisms are normally found on human eyelashes. Applying mascara on lashes has the potential to infect the mascara tube with microorganisms. Constant use of eye cosmetics may also affect tear film stability, resulting in decreased tear production. This pilot study in a real-world context looked at the microbiological contamination of two mascara brands worn daily for two months, as well as tear assessment before and after application. Methods and Materials: Sixty women aged 18 to 39 were randomly allocated to apply one of two brands of waterproof mascara or a non-waterproof brand on both upper and lower eyelids every day for two months. Microbial contamination for various Gram-positive and Gram-negative organisms was detected using the streak technique of culture and Gram staining after two months of mascara use. Tears were assessed before and after the first and second months of mascara usage, followed by a post-study questionnaire. Results: There was no growth of bacteria amongst waterproof mascara users. In non-waterproof users, Staphylococcus aureus growth was found in 33.3% of users, Micrococcus growth among 23.3% and both among 15.0%. Tear assessment showed a statistically significant difference between before and after the first month of mascara use and a highly statistically significant difference between before and after the second month of mascara use. Conclusion: Both waterproof and non-waterproof mascara are not safe for constant use.
Keywords: Bacteria, Cosmetics, Mascara, Microbial Growth, Tear Film
Abelson MB, McGarr PJ. Update on bacterial conjunctivitis. Available from: http://www/revophth.com/1998/rpj8ttops.html
Ahearn DG, Wilson LA. Microflora of outer eye and eye area cosmetics. Developments in Industrial Microbiology. 1976;17:23–28. Available from: https://www.cabidigitallibrary.org/doi/full/10.5555/19761331897
Silbert JA. Ulcerative bacterial keratitis. In: Anterior Segment Complications of Contact Lens Wear (2). (pp. 225-249) Butterworth-Heinemann. 2000.
Jeng BH, McLeod SD. Microbial keratitis. British Journal of Ophthalmology. 2003;87(7):805–806. Available from: https://doi.org/10.1136/bjo.87.7.805
Fleiszig SMJ, Evans DJ. Contact lens infections; can they ever be eradicated? Eye Contact Lens. 2003;29(1 Suppl):S67–S71. Available from: https://doi.org/10.1097/00140068-200301001-00019
Rope BL. Waterproof. Global Cosmet Industry. 2001;169(2):40–42. Available from: https://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1113539
Bergfield WF, Belsito DV, Jr. JGM, Andersen A. Safety of ingredients used in cosmetics. Journal of the American Academy of Dermatology. 2005;52(1):125–132. Available from: https://doi.org/10.1016/j.jaad.2004.07.066
Wilson LA, Julian AJ, Ahearn DG. The survival and growth of microorganisms in mascara during use. American Journal of Ophthalmology. 1975;79(4):596–601. Available from: https://doi.org/10.1016/0002-9394(75)90798-9
BLR. Conquering contamination. Global Cosmet Industry. 2002;170(1):40–43. Available from: https://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13450690
BLR. Conquering contamination, Part II. Global Cosmet Industry. 2002;170(5):40–44. Available from: https://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&lang=en&idt=15349716
Rudometkin NJ, Wessels IF, Hedayi RS, Choe JE, Roeske RE. Culture plate temperature and delayed incubation effect on bacterial recovery. Cornea. 2003;22(8):746–753. Available from: https://doi.org/10.1097/00003226-200311000-00007
Safe use of cosmetics. University of Illinois Eye Center. Available from: http://www.uic.edu/com/eye/LearningAboutVision/EyeFacts/Cosmetics.shtml
Rakow PL. Cosmetic use caveats. Journal of ophthalmic nursing & technology. 1988;7(6):224–226. Available from: https://pubmed.ncbi.nlm.nih.gov/3199454/
Mah-Sadorra JH, Yavuz SGA, Najjar DM, Laibson PR, Rapuano CJ, Cohen EJ. Trends in contact lens-related corneal ulcers. Cornea. 2005;24(1):51–58. Available from: https://doi.org/10.1097/01.ico.0000138839.29823.57
Schaefer F, Bruttin O, Zografos L, Guex-Crosier Y. Bacterial keratitis: a prospective clinical and microbiological study. British Journal of Ophthalmology. 2001;85(7):842–847. Available from: https://doi.org/10.1136/bjo.85.7.842
Capaldi PM. When should I replace my mascara? Contact Lens Forum. 1988;13(7):9. Available from: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=When+should+I+replace+my+mascara%3F+Contact+Lens+Forum&btnG=
Bourcier T, Thomas F, Borderie V, CC, Laroche L. Bacterial keratitis: predisposing factors, clinical and microbiological review of 300 cases. British Journal of Ophthalmology. 2003;87(7):834–838. Available from: https://doi.org/10.1136/bjo.87.7.834
Wilson LA, Ahearn DG. Pseudomonas-induced corneal ulcers associated with contaminated eye mascaras. American Journal of Ophthalmology. 1977;84(1):112–119. Available from: https://doi.org/10.1016/0002-9394(77)90334-8
Pack LD, Wickham MG, Enloe RA, Hill DN. Microbial contamination associated with mascara use. Optometry - Journal of the American Optometric Association. 2008;79(10):587–593. Available from: https://doi.org/10.1016/j.optm.2008.02.011
LAW, Kuehne JW, Hall SW, Ahearn DG. Microbial contamination in ocular cosmetics. American Journal of Ophthalmology. 1971;71(6):1298–1302. Available from: https://doi.org/10.1016/0002-9394(71)90979-2
Abdelaziz AA, Ashour MS, Hefni H, El-Tayeb OM. Microbial contamination of cosmetics and personal care items in Egypt - eye shadows, mascaras and face creams. Journal of Clinical Pharmacy and Therapeutics. 1989;14(1):21–28. Available from: https://doi.org/10.1111/j.1365-2710.1989.tb00217.x
Gefken C. Keeping cosmetics-drugs FDA compliant. Global Cosmetic Industry. 2005;173(4):64–65.
Guide to inspections of cosmetic product manufacturers. Available from: http://www.fda.gov.ora/inspect_ref/igs/cosmet.html
Perry B. Cosmetic microbiology. Microbiology Today. 2001;28(1):185–187. Available from: https://www.scribd.com/document/229040750/Cosmetic-mirobiologi
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