Volume: 2 Issue: 1
Year: 2025, Page: 46-51, Doi: https://doi.org/10.71325/ajjms.v2i1.25.7
Received: Feb. 8, 2025 Accepted: March 25, 2025 Published: April 19, 2025
Introduction and Aim: Both vitamin B12 (cobalamin) and vitamin B9 (folate) are the essential micronutrients for the origin of red blood cells, DNA synthesis, and overall haematological health. Although disorders like megaloblastic anaemia can result from vitamin deficiencies, little is known about how these vitamins affect haematological parameters. This study was aimed to evaluate the role of haematological markers in predicting vitamin B12 and B9 status among females aged 18-40 years. Methods: Using laboratory records from 150 female patients at Kasturba Hospital in Manipal from 2018 to 2022 a retrospective cross-sectional analysis was carried out. Serum levels of vitamin B12 and B9, iron profile values, and complete blood count were examined. Reference ranges were used to classify the data into three groups: deficient, normal, and above-normal. Jamovi software was used to conduct statistical studies, including chi-square tests, with significance set p< 0.05. Results: Of the group, 37.7% had a folate insufficiency and 42.7% had a vitamin B12 deficiency. Thirty percent had low haemoglobin levels, and forty-four percent had a lower mean corpuscular volume (MCV). Haematological indicators such as haemoglobin, RBC count, WBC count, and MCV did not display a statistically significant correlation with vitamin B12 status, according to Chi-square analysis (p>0.05). Conclusion: It emphasizes the necessity of routine screening and early management by pointing out the significant frequency of vitamin B12 and B9 deficiencies in the group under investigation. To better understand the relationship between haematological indicators and vitamin status, future studies should take into account co-morbid diseases, dietary practices, and genetic predispositions.
Keywords: Haematological markers; Vitamin B12 status; Folate (vitamin B9) status; Nutritional deficiencies
O’leary F, Samman S. Vitamin B12 in health and disease. Nutrients. 2010;2(3):299–316. Available from: https://doi.org/10.3390/nu2030299
Più LD. Characterization and exploiment of Microbial Strains to be used in Meat Processing and Fermentation. Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze e biotecnologie degli alimenti thesis
Tejaswini A, Harivarsha P, Sharan J, Chaithanya A, Guadalupe TT, Sandeep SL, et al. Role of Vitamin B12 and Folate in Metabolic Syndrome. Cureus. 2021;13(10):1–15. Available from: https://doi.org/10.7759/cureus.18521
Azzini E, Raguzzini A, Polito A. A Brief Review on Vitamin B12 Deficiency Looking at Some Case Study Reports in Adults. International Journal of Molecular Sciences. 2021;22(18):1–15. Available from: https://doi.org/10.3390/ijms22189694
Selhub J, Miller JW, Troen AM, Mason JB, Jacques PF. Perspective: The High-Folate–Low-Vitamin B-12 Interaction Is a Novel Cause of Vitamin B-12 Depletion with a Specific Etiology—A Hypothesis. Advances in Nutrition. 2022;13(1):16–33. Available from: https://doi.org/10.1093/advances/nmab106
Ballard HS. The hematological complications of alcoholism. Alcohol Health and Research World. 1997;21(1):42–52. Available from: https://pubmed.ncbi.nlm.nih.gov/15706762/
Singla R, Garg A, Surana V, Aggarwal S, Gupta G, Singla S. Vitamin B12 deficiency is endemic in Indian population: a perspective from North India. Indian Journal of Endocrinology and Metabolism. 2019;23(2):211–214. Available from: https://doi.org/10.4103/ijem.IJEM_122_19
Padmanabhan N, Menelaou K, Gao J, Anderson A, Blake GE, Li T, et al. Abnormal folate metabolism causes age‐, sex‐ and parent‐of‐origin‐specific haematological defects in mice. Journal of Physiology. 2018;596(18):4341–4360. Available from: https://doi.org/10.1113/JP276419
Koury MJ, Ponka P. New insights into erythropoiesis: the roles of folate, vitamin B12, and iron. Annual Review of Nutrition. 2004;24(1):105–131. Available from: https://doi.org/10.1146/annurev.nutr.24.012003.132306
Arndt MB, Abate YH, Abbasi-Kangevari M, Abd-Elhafeez S, Abdelmasseh M, Abd-Elsalam S, et al. Global, regional, and national progress towards the 2030 global nutrition targets and forecasts to 2050: a systematic analysis for the Global Burden of Disease Study 2021. The Lancet. 2024;404(10471):2543–2583. Available from: https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(24)01821-X/fulltext
Green R. Indicators for assessing folate and vitamin B-12 status and for monitoring the efficacy of intervention strategies. American Journal of Clinical Nutrition. 2011;94(2):666–672. Available from: https://doi.org/10.3945/ajcn.110.009613
Azimi S, Faramarzi E, Sarbakhsh P, Ostadrahimi A, Somi MH, Ghayour M. Folate and vitamin B12 status and their relation to hematological indices in healthy adults of Iranians: Azar cohort study. Nutrition and Health. 2019;25(1):29–36. Available from: https://doi.org/10.1177/0260106018815392
Alfthan G, Laurinen MS, Valsta LM, Pastinen T, Aro A. Folate intake, plasma folate and homocysteine status in a random Finnish population. European Journal of Clinical Nutrition. 2003;57(1):81–88. Available from: https://doi.org/10.1038/sj.ejcn.1601507
Fayet-Moore F, Petocz P, Samman S. Micronutrient status in female university students: iron, zinc, copper, selenium, vitamin B12 and folate. Nutrients. 2014;6(11):5103–5116. Available from: https://doi.org/10.3390/nu6115103
Lim HS, Heo YR. Plasma total homocysteine, folate, and vitamin B12 status in Korean adults. Journal of Nutritional Science and Vitaminology. 2002;48(4):290–297. Available from: https://doi.org/10.3177/jnsv.48.290
Macfarlane AJ, Greene-Finestone LS, Shi Y. Vitamin B-12 and homocysteine status in a folate-replete population: results from the Canadian Health Measures Survey. American Journal of Clinical Nutrition. 2011;94(4):1079–1087. Available from: American Journal of Clinical Nutrition
Selhub J. Homocysteine metabolism. Annual Review of Nutrition. 1999;19:217–246. Available from: https://doi.org/10.1146/annurev.nutr.19.1.217
Shams M, Homayouni K, Omrani GR. Serum folate and vitamin B12 status in healthy Iranian adults. EMHJ-Eastern Mediterranean Health Journal. 2009;15(5):1285–1292. Available from: https://pubmed.ncbi.nlm.nih.gov/20214143/
Xavier JM, Costa FF, Annichino-Bizzacchi JM, Saad STO. High frequency of vitamin B12 deficiency in a Brazilian population. Public Health Nutrition. 2010;13(8):1191–1197. Available from: https://doi.org/10.1017/s1368980009992205
Fakhrzadeh H, Ghotbi S, Pourebrahim R, Nouri M, Heshmat R, Bandarian F, et al. Total plasma homocysteine, folate, and vitamin b12 status in healthy Iranian adults: the Tehran homocysteine survey (2003–2004)/a cross – sectional population based study. BMC Public Health. 2006;6(1):1–8. Available from: https://dx.doi.org/10.1186/1471-2458-6-29
Golbahar J, Hamidi A, Aminzadeh MA, Omrani GR. Association of plasma folate, plasma total homocysteine, but not methylenetetrahydrofolate reductase C667T polymorphism, with bone mineral density in postmenopausal Iranian women: a cross-sectional study. Bone. 2004;35(3):760–765. Available from: https://doi.org/10.1016/j.bone.2004.04.018
Sivaprasad M, Shalini T, Balakrishna N, Sudarshan M, Lopamudra P, Suryanarayana P, et al. Status of Vitamin B12 and Folate among the Urban Adult Population in South India. Annals of Nutrition and Metabolism. 2016;68(2):94–102. Available from: https://dx.doi.org/10.1159/000442677
Mishra R, Madduri M, Pradhan S. Megaloblastic anemia in children from Eastern Odisha, India: A clinical and hematological profile analysis. National Journal of Physiology, Pharmacy and Pharmacology. 2022;12(9):1493–1496. Available from: https://dx.doi.org/10.5455/njppp.2022.12.01034202220012022
Bentley S, Hermes A, Phillips D, Daoud YA, Hanna S. Comparative effectiveness of a prenatal medical food to prenatal vitamins on haemoglobin levels and adverse outcomes: a retrospective analysis. Clinical therapeutics. 2011;33(2):204–210. Available from: https://doi.org/10.1016/j.clinthera.2011.02.010
Shamim AA, Kabir A, Merrill RD, Ali H, Rashid M, Schulze K, et al. Plasma zinc, vitamin B(12) and α-tocopherol are positively and plasma γ-tocopherol is negatively associated with Hb concentration in early pregnancy in north-west Bangladesh. Public Health Nutrition. 2013;16(8):1354–1361. Available from: https://doi.org/10.1017/s1368980013000475
Solomon LR. Cobalamin-responsive disorders in the ambulatory care setting: unreliability of cobalamin, methylmalonic acid, and homocysteine testing. Blood. 2005;105(3):978–985. Available from: https://doi.org/10.1182/blood-2004-04-1641
Elzen WPD, Weele GMVD, Gussekloo J, Westendorp RG, Assendelft WJ. Subnormal vitamin B12 concentrations and anaemia in older people: a systematic review. BMC geriatrics. 2010;10:1–11. Available from: https://doi.org/10.1186/1471-2318-10-42
Jenssen HB, Torsvik I, Ueland PM, Midttun Ø, Bjørke-Monsen AL. Biochemical signs of impaired cobalamin function do not affect hematological parameters in young infants: results from a double-blind randomized controlled trial. Pediatric Research. 2013;74:327–332. Available from: https://doi.org/10.1038/pr.2013.101
Smelt AF, Gussekloo J, Bermingham LW, EA, ADD, Eussen SJ, et al. The effect of vitamin B12 and folic acid supplementation on routine haematological parameters in older people: an individual participant data meta-analysis. European Journal of Clinical Nutrition. 2018;72(6):785–795. Available from: https://doi.org/10.1038/s41430-018-0118-x
Convertino VA. Blood volume response to physical activity and inactivity. American Journal of the Medical Sciences. 2007;334(1):72–79. Available from: https://doi.org/10.1097/maj.0b013e318063c6e4
© 2025 Published by Laxmi Memorial Education Trust. This is an open-access article under CC BY 4.0 license. (https://creativecommons.org/licenses/by/4.0/)
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