AJ Journal of Medical Sciences

Volume: 2 Issue: 4

  • Open Access
  • Original Article

In-Silico Systems Biology Analysis of the Klebsiella spallanzanii Defensome Against Bacteriophages

Aaina Ashraf1, Adreeka Jeet1, Maria Raju1, M A Prathiksha1, Mylavarapu Sudeepthi1, C Sandeep Kumar2*

1B.Sc. Students, Department of Biotechnology and Genetics, School of Sciences, Jain (Deemed-to-be University), Bengaluru, Karnataka, India. 
2Assistant Professor, Department of Biotechnology and Genetics, School of Sciences, Jain (Deemed-to-be University), Bengaluru, Karnataka, India.
 

* Corresponding author.
C Sandeep Kumar
[email protected]

Year: 2025, Page: 163-169, Doi: https://doi.org/10.71325/ajjms.v2i4.2 5.64

Received: Oct. 17, 2025 Accepted: Dec. 4, 2025 Published: Dec. 31, 2025

Abstract

Introduction: A common source of nosocomial infections, Klebsiella pneumoniae has a high morbidity and fatality rate, particularly in immunocompromised patients and infants. Public health is seriously threatened by its multidrug-resistant (MDR) strains. Despite therapy, the death rate from hospital-acquired pneumonia caused by K. pneumoniae is higher than 50%. The newly discovered species Klebsiella spallanzanii (Ko3 phylogroup) and K. oxytoca have recently become clinical concerns. Bacteriophage therapy is a promising alternative in light of the growing antibiotic resistance, however its effectiveness may be hampered by bacterial defence mechanisms. In order to improve phage therapy tactics, this work attempts to describe the defence mechanisms of K. spallanzanii. Methods: The three webservers PADLOC, CRISPRCasFinder, and DefenseFinder were used to analyse the defence mechanisms of Klebsiella spallanzanii, which was isolated from a Chinese patient suffering from urinary tract infections. DefenseFinder checks for a variety of defence mechanisms, CRISPRCasFinder finds CRISPR-Cas systems, and PADLOC finds prokaryotic antiviral defence systems. These methods were used to map and categorise the strain's defence mechanisms using genomic data. Results: Klebsiella spallanzanii has a strong anti-phage arsenal, as evidenced by the discovery of a CRISPR-Cas system and seven other defence mechanisms, including as restriction-modification, abortive infection, and toxin-antitoxin systems. Interpretation & conclusions: The discovery of seven additional defence systems, including CRISPR-Cas, indicates that K. spallanzanii has a variety of defence mechanisms against phage invasion, which may limit the effectiveness of bacteriophage therapy. These results demonstrate how intricate bacterial defences are, requiring specialised phage designs to avoid or interfere with these systems.

Keywords: <I>Klebsiella pneumoniae</I>, <I>K. oxytoca</I>, <I>Klebsiella spallanzanii</I>, Immunocompromised individuals, PADLOC webserver, CRISPRCasFinder

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Cite this article

Ashraf A, Jeet A, Raju M, Prathiksha MA, Sudeepthi M, Kumar CS.  In-Silico Systems Biology Analysis of the Klebsiella spallanzanii Defensome Against Bacteriophages. AJ J Med Sci 2025;2(4):163-169

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